Three-dimensional structure of a sarcoplasmic calcium-binding protein from Nereis diversicolor.
نویسندگان
چکیده
منابع مشابه
Amino acid sequence of a sarcoplasmic calcium-binding protein from the sandworm Nereis diversicolor.
Muscles of invertebrate species contain abundant quantities of soluble, sarcoplasmic, high affinity Ca2+-binding proteins (SCBPs). The SCBPs belong to the calmodulin superfamily and contain four homologous domains (I-IV) which arose by reduplication of a gene for a small ancestral protein. We have determined the amino acid sequence of the SCBP from the sandworm Nereis diversicolor. This protein...
متن کاملCrystallization and preliminary x-ray investigation of sarcoplasmic calcium-binding protein from Nereis diversicolor.
Crystals of sarcoplasmic calcium-binding proteins from Nereis diversicolor have been grown from solutions of ammonium sulfate. The crystals are monoclinic, space group P2(1); the axes are a = 43.65 (1), b = 56.05 (1), c = 65.77 (1) A, and beta = 92.58 (2) degrees. The crystals are quite stable to x-rays and diffract beyond 2.5 A resolution. The asymmetric unit contains two protein molecules.
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The sarcoplasmic calcium-binding protein (SCP) of the sandworm Nereis possesses three Ca2(+)-Mg2+ sites but no Ca2(+)-specific site. Binding of Mg2+, but not of Ca2+, displays a marked positive cooperativity. The apparent cooperativity of Ca2+ binding in the presence of Mg2+ results from the allostery in Mg2+ dissociation. Binding of the first Ca2+ or Mg2+ induces all the conformational change,...
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Although Beadle a9 early as 1937 suggested that the urine of Nereis diversicolor O. F. Miiller might be of lower concentration than the body fluids when this worm is hyperregulating in low salinities, there has been no direct measurement of urine concentration to support this reasonable idea. Jorgensen & Dales (1957) studied osmotic water uptake and loss in N. diversicolor and calculated that t...
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Animals are exposed, in the natural environment, to a range of human activity related dissolved chemicals that are potentially impacting on their fitness. The animals responses to such stress determines its fitness, physiology and in case of chemical signals also its behaviour. If physical or chemical changes in the ecosystem make the detection of such a chemical impossible, then it can be reas...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1991
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)52484-4